Yeast Culture in Conditions of Limited Reproduction, Added Food and Predation (1700 words)

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Lab Report Outline

Yeast Culture Lab

I. Introduction

1. Briefly introduce the background information of this lab. Define reproduction, consumption, and death in population.
Explain the main purpose of the experiment, such as what you will accomplish during the experiment and why it is important to conduct this lab.

2. State your hypothesis of this lab.
Your hypothesis must be in an if–then format and include a comparison of at least one variable to the control.
Then, in 1 or 2 sentences, explain what you expect will happen, and include at least one alternative outcome.

II. Material and Methods

1. List equipment and materials you will use. Cite any worksheets or other reference materials you use.
2.In your own words, create a detailed and numbered list of the steps necessary to complete the experiment.
Only include the steps YOU take to complete the lab assignment.
You do NOT need to discuss the aspects of the actual laboratory experiment simulation, such as yeast culturing and temperatures.

III. Results

1. List any questions you have about your results.
2. Make both qualitative and quantitative observations.
3. Show your work and remember to include necessary units. Include graphs, when appropriate.
4. Include tables or graphs to summarize your results, if necessary.

IV. Discussion

1. Explain the shape of the population graph for each condition. Why does the graph peak before declining? How do the limiting factors in this experiment affect carrying capacity?
2. What are the phases of the population growth cycle? How does using a simple organism allow for the study of a more complex population?
3. Describe a biogeochemical cycle that had an effect on the yeast cultures by outlining the flow of energy in the cycle.
4. Identify and examine the food chain in a yeast community.

V. Conclusion

1. Accept or reject your hypothesis.
2. State any discrepancies; these are problems or unpredicted events that occurred. These errors may be due to equipment or human error.
3. Based on the lab and your weekly readings, discuss the challenges of studying populations over time.
4. Make suggestions for improvement or for further research.

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Yeast Culture in Conditions of Limited Reproduction, Additional Food and Introduced Predation

Introduction

Reproduction is the biological process by which new individual organisms called “offsprings” are produced from or by the existing organisms called “parents”.
Reproduction can be asexual (such as simple cell division observed in unicellular organisms, but also cloning of both unicellular and multicellular organisms) and sexual, depending on whether the process requires the sexual interaction, which enables the combination of the parental genetic material, usually through, but not limited to, the merging of specialized (typically haploid) cells called “gametes”.

If the reproduction requires sexual interaction, it is sexual; if the process does not involve any type of sexual interaction, it is asexual.
Yeast (Saccharomyces cerevisiae) is a unicellular eukaryote which can exist both in haploid and diploid mode.

All yeast cells can reproduce asexually, however, mating of yeast occurs exclusively between haploids, called spores.
There are two types of spores: “a” and “α”, which mate with each other. Spores of the same type cannot mate among themselves.
Sexual differentiation of diploid cells occurs only under stressful conditions, such as nutrient depletion (Cap, Vachova & Palkova, 2009, 2010).

In such conditions, a diploid cell undergoes meiosis to produce haploid spores (the process is called “sporulation”) – two “a” spores and two α spores, four in total, which can go on to mate in a process called “conjugation”. a cells produce a-factor, a mating pheromone, which attracts the neighboring α cells. a cells respond to α-factor, the α cell pheromone, by growing a projection, called...
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