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1. SIM test media is a complex three-part test that tests at the same time the presence of hydrogen sulfide, indole, or the mobility of the micro-organism. If we do not want to use the sulfide portion of the test, than we can omitted from the media - sodium thiosulfate (as part of the substrate) and ferric ammonium citrate (as an indicator). The above-mentioned ingredients are necessary to identify the occurrence of hydrogen sulfide.
Their absence can make it difficult for us identifying Salmonella spp and its differentiation from Escherichia spp, which requires us great caution in the indole part of the test, which will then be crucial....
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