Question

1. If different concentrations of the same analyte are introduced into the mass spectrometer, how will the spectra differ? How will they be the same?

2. In EI, the molecular ion of a compound has a 80% relative abundance. If the relative abundance of the [M + 1] ion is 12 %, how many carbons are in the compound?

3. If a mass spectrum has the molecular ion in high abundance, what type of functional group(s) would you expect it to contain?

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1. If different concentrations of the same analyte are introduced into the mass spectrometer, how will the spectra differ? How will they be the same?

Ans. Spectra will differ only in case where molecular ion peak is not visible at less concentration but it could be visible when concentration increases and impurity of sample can also be easily detected with increased concentration of analyte otherwise mass spectra of same analyte would be same at different concentrations because y-axis of spectra shows relative abundance peak of ions that will not vary with change in concentration and x-axis shows mass of ions that is also not related with change in concentration....

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