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1. Which compound would you expect to have a shorter retention time on a reverse-phase HPLC column with a water/acetonitrile mixture adjusted to pH 3 with acetic acid — benzylalcohol or benzoic acid?

Ans: Benzoic acid is expected to have a shorter retention time compared to benzylalcohol on a reverse-phase HPLC column with a water/acetonitrile mixture adjusted to pH 3 with acetic acid. Because of benzoic acid is more polar than benzyl alcohol and reverse phase HPLC column [in which stationary phase is nonpolar and mobile phase is polar in nature, thus adsorb the non-polar or less polar compound (here benzyl alcohol) with higher affinity than more polar compound (here benzoic acid)] with polar solvents such as water/acetonitrile and in acidic condition favors the fast elution of benzoic acid than benzyl alcohol....

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