These are the questions that need to be answered for the paper "New Compound Sets Identified from High Throughput Phenotypic Screening Against Three Kinetoplastid Parasites: An Open Resource"
1. What is the context of the paper-background
2. Summarize big picture aspect-impact
3. What is the central hypothesis to be tested
4. FOR each figure-what are the conclusions based upon?
5. What are the controls used in each figure?
6. What is WRONG with the data/interpretation?
7. What experiment would you do to check?
8. What are the major conclusions/results
9. What other experiments do the authors propose?
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1. What is the context of the paper-background?
Leishmania and Trypanosoma are two genera of single-celled parasites that cause a variety of human diseases including leishmaniasis, Chaga’s disease and African sleeping sickness. It is estimated that each year ½ billion people are threatened by these parasites posing a significant cost to lives lost, chronic illness and degraded quality of life. However, drugs for treatment of these parasite infestations are unavailable or largely ineffective.
These parasites share several biological features in common including being single celled parasites with a life cycle requiring a human host alternating with an insect host, a single flagellum, and a large mitochondrion containing a DNA Kinetoplast. Comparison of the genomes shows that these parasites each possess more than 8,000 genes and approximately ¾ of these genes are known orthologs of genes in other organisms. Also approximately 50% of the potential proteins coded by these genes are not found in humans providing many potential protein targets for drug treatment.
Drug companies maintain large libraries of chemicals that are potential candidates for disease treatment. When trying to find candidates to treat particular diseases or disorders these chemical libraries can be tested for potential activity against the target disease vector or activity in the disease process. The current study uses a library of 1.8 million chemicals maintained by GlaxoSmithKline in a High Throughput Screening (HTS) assay to test for activity against Leishmania and Trypanosoma parasites. No other pharmaceutical company chemical library screen against these parasites has ever been published....
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