The given paper is "The Pseudomonas aeruginosa Periplasmic Protease CtpA Can Affect Systems That Impact Its Ability To Mount Both Acute and Chronic Infections"

1. What is the context of the paper-background
2. Summarize big picture aspect-impact
3. What is the central hypothesis to be tested
4. FOR each figure-what are the conclusions based upon?
5. What are the controls used in each figure?
6. What is WRONG with the data/interpretation?
7. What experiment would you do to check?
8. What are the major conclusions/results
9. What other experiments do the authors propose?

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1. What is the context of the paper-background

Pseudomonas aeurginosa is a gram negative bacteria that is not innately disease causing but is an opportunistic pathogen and can cause acute as well as chronic infections in the general population as well as in nosocomial situations. It is especially troublesome in cystic fibrosis patients where it can cause chronic infections in the lungs and covert to a mucoid form causing thick mucus accumulation and thus negative complications.

One of the primary means of pathogenesis of P. aeruginosa during acute infection is facilitated through the production of proteins for exocytosis (especially through Psc type 3 secretion (T3SS). Protease production is of particular importance and is thought to play a primary role in conversion of the bacterial colony to the mucoid form in chronic lung infections in CF patients. Mucus production is usually inhibited by the anti-sigma factor, MucA (among others) which is attacked by particular proteases when the bacteria is under stress thus facilitating conversion to the mucoid form. One protease identified in E. coli that is involved in MucA destruction is the carboxy-terminal protease (CPT) prc (coded by gene PA3257). These proteases that target the c-terminal end of proteins are little studied.

In P. aeruginosa two CTP’s have been identified in the genome, PA3257/Prc and PA5134/CtpA. CtpA is the subject of this study and shares some characteristics in common with Prc in that it has been shown to be periplasmic and of similar molecular weight (30kDa less than Prc)....

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