l\[ATCI UNG. Choose the item in column 2 that best matches each ite...

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l\[ATCI UNG. Choose the item in column 2 that best matches each item in column 1. Items in column 2 may be used multiple times. l) Pnwide resist.i.nce to cumpression. 2) Provide high tensile strength in the extracellular m11trix. 3) PrO\·ide stretch to the extrJcelhil.i.r m.i.trix. A) lntennedi,ite fil.i.ments B) Elilstin C) Clyrnsaminoglycans D) Coll.igen l\1Ul TIPlE CHOICE. Choose the one alternative that best completes the statement or answers the question. ➔) Dming metaph.i.se, polar ejection is a cieved by ____ binding _ ___ proteins. A) plasma membrane, dynein B) aster, dynein C) centromere, myosin D) kinetochore, \fAD2 E) D\:A, kinesin 5) Laminin binds to: A) lntegrins B) Laminin C) nucle.i.r lamins D) the ER E) Two of the ab01.:e 14) Which white blood cell surface molecules play a major role in allowing them to stay in contact with the endothelial cells lining the blood \·essel? A) Selectins B) '.\'CA\ls C) ICA~ls D) Oligosaccharides E) Cadherins 1 ?) Ana phase A incl lid.es which microtubule-dependent process? A) the separation of spindle poles using astral and interpolar microtubules B) the alignment of chromosomes along the equator C) the formation of a contractile belt along the cell equator D) the dissolution of centrosomes and formation of spindle poles at each end of the cell E) the shrotening of kinetochore microtubules 16) Anaphase B refers to \,·hich microtubule-dependent process? A) the shortening of kinetochore microtubules B) the formation of a contractile belt along the cell equator C) the alignment of chromosomes along the equator D) the dissolution of centrosomes and formation of spindle poles at each end of the cell E) the separation of spindle poles using astral and interpolar microtubules 17) Cohesin molecules are proteolyzed following the destmction of \,·hich other protein?: A) securin B) separase C) caspase-9 D) cdc20 E) SCF 18) Which of the following must occur in interphase for cell di\·ision to proceed? A) D'.\"A replication B) D:\A conden:,ation C) APC actirntion D) centrosome replication E) t\\'0 of the abm·e 19) During prometaphase, the nuclei.1r en\'elope disintegrates due tl) A) hydrolysis of A 1 r on actin microfilaments B) ubiquitination of nucleoporin complexes C) proteolysis of the cytoskeleton D) phosphorylation of Jamin proteins E) all of the ab,we 20) The initial minus end directed sliding of luterally attached kinetochores may aid in detection of ____ and help ensure that a centromere is attached to each ___ _ A) tension, spindle pole B) attachment, kinesin C) attachment, dynein D) f!e'\ibility, telomere E) Ca2+, astral microtubule 26) Cdk activity is regulated in all the following \\'ay:, EXCEPT: A) phosphorylation of cdks by CA Ks B) dephosphorylation of cdks b) cdc25 C) phosphorylation of Cdks by \\'ee-1 kinase D) ubiquitination and proteolysis of the Cdk-a-,..,ociated cyclin protein E) ubiquitination and proteolysis of the Cd!-. protein 27) SCf and ~ID\12 are examples of A) E1 ubiquitin ligases B) GTPase activating proteins C) serine/threonine phosphatases D) cyclins E) ATPases 2 ) A number of proteins ha\·e the acron) m "cdc . These proteins all earned this name because they \\'ere d1scm ered in a ___ screen of ___ for genes that interfere \\'ith the "cell dinsion cycle" A) genetic, yeast B) biochemical, mice C) immunological, bacteria D) \"isual, \'iruses E) molecubr, billikens 29) A genetic engineenng project ha,; resulted in cells that lac!-. the protein Arf. 1hese cells become cancerou.., at a high rate, compared to controls, when subjected to a D'\A damage inducing compound? l his is because Arf L1sually acts a~ a: A) D:S-:A rep.1ir enzyme B) Proto-oncogene C) Tumor supressor D) Chromophore E) Dng bone 30) An ind1\·idual caspase protein is act1\·ated b~ A) phosphoryl.1tion B) binding to PIP2 C) binding to ATP D) proteolysis E) binding to GTP 31) \\'hich of the signaling intermediate enzymes plays a m.1jor role in gro\\'th signab? A) '.':O synthase B) Pho~pholipase C C) cG\!P phosphodiesterase D) Pl 3-kinase E) aden) I) I cyclase 32) The role Bl 13-only domain proteins play in apoptosis is to A) trigger exocytosis of BHl -3 B) inhibit BH 1--l domain proteins C) form dimers \\'ith caspases, creating large pores in the nuclear membrane D) bind to Rb, promoting G L cyclm actirntion E) aggregate to allm,· release of cytochrome C -10) To propel the movement of cilia, ___ spans beh,een __ _ A) Kinesins, triplet microtubules B) Myosins, actin filaments C) Dyneins, doublet microtubules D) i'. lyosins, basal bodies E) Dyneins, triplet microtubules -ll) The difference ben,·een a sarcoma, a carcinoma, and leukemia 1s A) whether the cells ha\'e been exposed to CV - irradiation B) what tissue the cancer originated in C) ,vhether the cancer cells lack p53 or not D) whether the cancer is malignant or benign E) how many cells are present in the tumor .Q) Centriole replication happens at the same time as: A) Cell growth of G I B) D\:A replirntion C) Cell growth of G2 D) ~litosis and separation of chromosome,, E) Cytokinesis and separation of the cytoplasm -13) \\'hat happens to ~IAD2 when it asssociates with a kinetochore? A) it is conYerted to an APC inhibitol") form B) it is degraded C) it n uoresces D) it promotes cytokinesis E) it depolymerizes microtubules -1-l) The POTE:\"TIAL for Ras and ~lye to dri\·e EXCESS cell proliferation classifies them a,,: A) Proto-oncogenes B) Growth factors C) Tumor suppressors D) Adapter proteins E) Caspases -15) Cell di, ision is controlled in two" a):,,. First b} gene, that promote cell di, i,ion. and second b) genes that monitor an<l suppress cell di, ision. This supports the finding that: A) Cancer can be benign or malignant B) Different cancers arise from different tissues C) Multiple mutations are required for cancer D) Cancer can become im asi\ e E) Benign tumor~ are usual!} a compact cell mass Part B: Cumulative Your signature on this exam is your pledge that you have abided b; in taking this exam, and have used no dishonest or deceptive methods to obtain a better grade. Your exam will not be accepted or graded without your signature. Printed Name: _ _______ _ _ _______ _ Signature: MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. ~ 1 1) Ubiquitylation drives unidirectional progression of the cell cycle. is not targeted: Which of the following A) Gl/S cyclin B) S cyclin C) M 9rclin --- ------- D) cdc6 E) Cyclin dependent kinase C:f '2) Which of the following is NOT created during production of ATP in mitochondria? A) 02 B) FADH2 C) ADH D) H20 E) CO2 11) Which of the following provides resolution of the smallest cellular objects? A) deconvolution microscopy B) confocal microscopy C) scanning electron microscopy D) differential intererence contrast (DIC) microscopy E) transmission electron microscopy bz,,, 12) Most transmembrane receptors that send signals across the plasma membrane contain: A) cytosolic beta sheets B) a beta barrel C) an amphipathic alpha helix D) a transmembrane alpha helix E) none of the above &> 13) Phospholipid bilayers assemble because: A) hydrocarbon chains form bonds with one another B) polar phospholipid head groups form ionic bonds with one another C) phospholipids hydrocarbon chains minimize their contact with water D) cholesterol molecules provide linkages between hydrocarbon chains E) all of the above bf 14) Which of the following is true of the lysosome? A) it has a very basic pH B) it degrades only glycoylated proteins C) it degrades only ubiquitinated proteins D) if its membrane were ruptured, it would degrade everything in the cell E) it degrades proteins, nucleic acids, and lipids & t; 15) Why are glycoproteins found primarily on the extracellular surface of the plasma membrane? A) The enzymes that add sugar groups are present only in the lumens of the endoplasmic 1 eticnlum and the Golgi-appara-tus-:- 8) Sugar groups are added by enzymes only found in regulated secretory vesicles destined for the plasma membrane. C) Glycoprotein sugar groups on the intracellular surface are cleaved off by enzymes found only in the cytosol. D) Glycoproteins flip spontaneously, after incorporation into the membrane, due to their hydrophilic sugar head groups. E) The enzymes that add sugar groups are present only on the extracellular surface of the plasma membrane. 7z. _. 22) Carbon fixation is A) the addition of CO2 to organic molecules to create sugar 7s 7G B) the donation of electrons from CO2 to the electron transport chain C) the donation of electrons to CO2 during photosynthesis D) the addition of water molecules to CO2 to create bicarbonate E) the creation of CO2 during respiration 23) How is the cell's pH maintained? A) import and export of H+ and HCO3- B) import and export of Na+ and CIC) opening of OH- channels in the membrane D) opening of H+ channels in the membrane E) all of the above 24) Which item below is bound by all of the folowing: cadherins, protein kinase C, and calmodulin. A) Ca2+ B)ATP C) Receptor Tyrosine Kinases D) G-Protein Coupled Receptors E) Heterotrimeric G-Proteins 25) The activity of adenylyl cyclase can be regulated by: A) Gt alpha B) Gi alpha C) Gs alpha D) two of the above E) all of the above 26) Transport of large proteins into the nucleus from the cy....,,,_,,'-""-"-~'1-"'-"~ '------------- A) chaperone proteins B) gated transport C) transmembrane transport D) vesicular transport E) all of the above 27) Which proteins must be translocated across the ER membrane? A) proteins in the lumen of the trans- Golgi network B) plasma membrane transmembrane proteins C) lysosomal hydrolases D) proteins secreted into the extracellular space E) all of the above 34) Members of the animal steroid hormone receptor family A) undergo autophosphorylation B) are transmernbrane proteins C) regulate sexual development D) are associated with the endoplasmic reticulum E) bind directly to DNA 35) Ras, Rab, Ran, and Rae are all what kind of molecules? A) G-protein coupled receptors B) receptor tyrosine kinases C) monomeric GTPases D) adaptor proteins E) heterotrimeric GTPases 36) A bacterium might use all the following to fuel active transport EXCEPT: A) transport down a H+ gradient B) transport down an electochemical gradient C) ATP D) transport down a a+ gradient E) absorption of light 1 o 40) If it contains no signal sequence, a protein is likely to reside in: 1/ A) the endoplasmic reticulum B) the cytosol C) the plasma membrane D) the nucleus E) the extracellular space 41) The phase of the cell cycle associated with the replication of D A is A) telophase. B) anaphase. C) prophase. D) metaphase. E) interphase. 42) R7 photoreceptor differentiation in the fly eye depends, most directly, on what kind of source cell to target cell signaling? A) contact dependent B) endocrine C) autocrine D) paracrine E) synaptic 43) An extracellular signal is transmitted into a cell through a receptor tyrosine kinases (RTK) when: A) extracellular ligand binding causes a conformational change in the cytosolic domain B) extracellular ligand binding stimulates proteolysis of the receptor C) extracellular ligand binding induces dissociation of a heterotrimeric G-protein D) extracellular ligand binding stabilizes the receptor as a dimer E) extracellularly bound ligand is trasported into the cell 44) What is one of the main functions of the P-type Na+/K+ pump? A) to maintain the osmotic pressure m the cen,------------------ B) to depolarize the membrane during action potentials C) to fuel transport of proteins into the nucleus D) to mediate transport of glucose across the plasma membrane E) to produce an acidic environment in the lysosorne 45) How is the intracellular Ca2+ concentration maintained? A) Ca2+ is passively transported into the lysosome by Ca2+/H+ antiports B) Ca2+ is transported to the proteasorne and degraded C) Ca2+-channels in the plasma membrane open to allow Ca2+ flow into the extracellular space D) Ca2+ is actively pumped into the ER by a Ca2+ ATPase E) Ca2+ is sequestered in the nucleus /oo 50) A group of cells is lysed in hypotonic solutions and the membranes are pelleted and analyzed by SDS-PAGE (as shown). Three major protein bands are observed (lane 1) with apparent molecular weights of 200kD, 120kD and 80 kD. Some of the pellet is subsequently washed with a high salt solution and the remaining proteins are also analyzed on by SDS-PAGE (lane 2). From the data, you can say with confidence that the 80 kD protein is: markl?f 1SO kD - 1SOU) - IOOW - 7HO - 5011[) - A) a peripheral membrane protein B) a protein with an amphipathic alpha helix C) a protein with a CPI-linkage D) a transmembrane protein E) an integral membrane protein rysate pelle: --- pelle1 after high-salt w.ish 2 - - 6) All of the follo\\'ing are p roteins in the extrc1cellular matrix EXCEPT: A) hepc1rin sulfate B)-elastin C) laminin D) collagen E) fibronectin MATCHING. Choose the item in column 2 that best matches each item in column 1. Items in column 2 may be used multiple times. 7) What process leads to stable p537 8) What \\'Ould happen initially if Rb were mutated and could not be phosphorylated? 9) What would increc1sed expre.:,;sion of telomerase in a cell be likely to cause? 10) A cell line \\'ith nonfunctional Apaf p rotein would be limited in its option.:,; for: A) D:\'A replication B) D\:A damage C) Apoptosis D) Cell cycle a rrest at G 1/5 E) Delilyed senescence r-.tUl TIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 11) The structme of collagen includes: A) tripeptide repeats of Gly-Pro-X B) many sulfate groups \\'ith a strong negative chilrge C) nucleation of plus end fibers D) t1,o hea1·y chilins and two light chJins E) beta- sheets 12) \\'hat property of glycosaminoglycans is essential for their role in connective tissue? A) They have an abundance of lysine residues B) They fold easily into compact structures C) They degrade collagen D) They attract \\'ater E) They are rapidly proteolyst>d 13) l.oss of myosin 11 \\'Ould have 1, hat effect on animal cell di\'ision? A) loss of cytokinesis B) loss of chromatid mo\'ement to\\'ard spindle poles C) loss of D\:A replication D) loss of astral microtubule mo1 ement toward the plasma membrane E) loss of nuclear membrane dissolution 21) The phragmoplast in plant cells is localized in the same plane as which structure in animal cells? A) the contractile belt B) the spindle pole C) the adhesion belt D) the nuclear lamins E) the astral microtubules 22) After analyzing a population of cells using a flow cytometer, \\'hich of the following graphs would most likely represent a mutant cell line with inactiYe cdc25 . Cf) A) B) C) -Q) 0 ~ 0 I... Q) ..0 E ::J z 1 2 1 2 1 2 DNA Content (Relative Units) A) B) C) 23) All of the fo llowing are phosphorylatcd by \l-cyclin/Cd1's EXCEPT: A) p53 B) APC C) nuclear lamins D) cdc25 E) microtubule- associated proteins 2-l) During Prophase, what is the largest barrier to mitotic progress A) .\1 cyclin not yet e,pressed B) D;\J A replication incomplete C) microtubule stability D) Inactive APC E) nuclear envelope 2:i) Imagine that you could micrninject cytochrome c into the cytosol of both wild-type cells and cells that wen.~ defective for both the BH'~ -only and the BH123 p rotein families (de.1th-promoting members of the Bcl-2 family). Would you e,pect one, both, or neither of the cell lines to undergo apoptosis? A) Only the defective cell line would undergo apoptosis B) Neither cell line would undergo apoptosis C) Both the wild-type and mutant cell lines woukl undergo apoptosis D) Only the wild-type cell line would undergo apoptosis 33) Papillomavirus is a major cause of cerdcal cancer. This \'irus acts by A) directly activating D~A polymerase B) seqL1estering Rb and p53 C) repressing \ ·(-cyclin degradation D) repressing 5-cyclin degradation E) seqllestering Ras GAP proteins 3-l) Which of the following molecules is associated with the process of "sorting out"? A) lgG Sllperfamily proteins B) Selectins C) Cadherin!> D) Occludins E) lntegrins 35) Which of the following is TRCE about fibronectin? A) fibronectin plays a key role anchoring cells to the basnl lamina B) fibronectin can activate and bind to integrins C) fibronectin RGD domains are a proline rich domnin for collagen binding D) fibronectin functions as a single protein chain E) fibronectin is a heterotrimeric protien 16) Which stnge of cancer progression is least problemntic for cancer cells? A) evading control of the cell cycle B) breaking out of the primary location C) surviving circulation D) growing\\ ithout the usual growth and sur\'h·nl factors E) expanding in a foreign location from a microtumor 37) Which of the follO\,mg often occurs when cells lad, p53? A) cell d1fferentintion B) arrest of cell cycle in GO C) apopto::.i::, D) cancer E) arrest of cell cycle in G l 18) \,\'hat is a defining difference between initiator and e-...ecutioner caspases? A) one has a scaffold docking domain. B) one has proteolytic activity C) one has a role in apoptosis D) one i!> s~ nthesized as a pro-enLyme E) one is made of protein 39) \,\'hen D;\A damage is detected, ChU/2 kinase activit~ can be used to phosphorylate: A) cdc25 B) actin C) Rb D) cdc6 E) Two of the above t\IATCHI G. Choose the item in column 2 that best matches each item in column 1. Items in column 2 may be used multiple times. -16) Assembled on cadherins -17) Can pre,·ent the movment of transmembrane proteins from the apical to the basal region of a cell. -18) Can interact with intermediate filaments and integrins. -19) Is created when two conncxons come into register. :iO) Are made of claudin and occludin proteins. A) Hemidesmosome B) Tight Junctions C) Desmosome D) Gap Junctions E) Plasmodesmata . ~ MATCHING. Choose the item in column 2 that best matches each item in column I. lj I 3) Non-polymerized subunits A) microtubules are loaded with ATP using profilin .. B) actin microfilaments 51_, 4) contained in the contractile C) intermediate filaments belt that divides animal cells during cytokinesis ~,'; 5) attaches to hemidesmosomes 15t 6) interacts with kinesin-5 during mitosis ~, 7) subject to dynamic instability MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 8) Action potentials are driven along the length of axons by A) opening of voltage-gated Na+-channels B) inhibition of the Na+-K+ pump C) binding of neurotransmitters all along the length of the axon D) opening of K+ leak channels E) all of the above I 9) What is a signal sequence? A) an amino acid sequence that directs a protein to a particular organelle B) a nucleotide sequence that provides a binding site for a transcription factor C) a nucleotide sequence that indicates where transcription of a gene begins D) an amino acid sequence that directs a protein toward proteolysis E) none of the above 10) All of the following are functions of the Golgi EXCEPT: A) initiating 0-linked glycosylation B) altering N-linked oligosaccharides C) degradation of proteins, lipids, and nucleic acids D) post-translational modifications such as glyoxylation E) sorting of proteins into regulated secretory vesicles 6h ..:l-'6j°Which of the following is FALSE regarding myosin-mediated skeletal muscle contraction? A) It is an ATP-dependent process B) It occurs synchronously due to T-tubules C) It occurs in small rnononucleate cells D) It is regulated by Ca2+ release E) Two of the above 17) All of the following help protect the cell from rnisfolded proteins, EXCEPT: A) signal recognition particles B) ubiquitin conjugating enzymes C) calnexin and calreticulin D) ubiquitin ligases E) proteasornes 18) Ubiquitin Ligases are important for: A) creating multivesicular bodies B) perceiving signals as receptors C) destroying misfolded proteins D) regulating cell cycle checkpoints E) all of the above 19) All of the following are important for cell movement EXCEPT: A) myosin-rnediated contraction B) severing of actin filaments by cofilin C) nucleation by ARP2/3 proteins D) rapid depolymerization of intermediate filaments E) rearrangement of focal adhesions lo 20) Hydropathy plots are useful for predicting: A) the presence of beta-sheets ----- B)7:he presence of nuclear import sequences C) the presence of mitochondrial signal sequences D) the presence of alpha helices E) the presence of transrnembrane domains 7 / 21) DNA damage induces cell cycle arrest by activating A) Cdks B) Ras C) p53 D)Myc E) Rb 7 e;J 28) All of the following use vesicular transport to import and export proteins and lipids EXCEPT: A) endoplasmic reticulum B) mitochondria C) lysosomes D) early endosomes E) Golgi 7q 29) In which circumstance is active transport always necessary? A) to move solutes from the intracellular space to the extracellular space B) to move a solute against its concentration gradient C) to move solutes from the extracellular space to the intracellular space D) to move charged molecules or ions across the plasma membrane E) all of the above 30) What change would decrease fluidity of a membrane? A) decreasing the percentage of unsaturated fatty acids in phospholipids B) increasing the temperature C) making the fatty acid chains shorter D) all of the above E) none of the above 31) Which of the following would be an example of an oncogene? A) Rb that is unable to be phosphorylated B) Ras that is unable to bind GTP C) Ras that is unable to bind a GEF D) p53 that is unable to be ubiquitinated E) myc that is overexpressed 32) Which statement about transporters and ion channels is true? A) transporters can only mediate active transport B) transporters move molecules across-the...membrane .. Jas.te.t-than-ion..channel"'----------- C) transporters can be open to both sides of the membrane at once D) ion channels can only mediate passive transport E) ion channels are always open 33) What is a lipid raft? A) a specialized region of the plasma membrane used in signaling B) the region of the mitochondrial inner membrane that contains ATP synthase C) the region on transport vesicles used for fusion with target membranes D) an area of the endoplasmic reticulum specialized for protein translocation E) an area of the trans-Golgi network used for protein sorting 37) A new kingdom of organisms is discovered, and found to contain abundant proteins that bind to TIP and then polymerize. Once in a polymer, these proteins hydrolyse TIP to TOP. The similarity to known cytoskeletal proteins prompts a study of polymerization kinetics. In vitro Ccs are given in the figure below. Surprisingly, when 1.5 uM free subunits are added to a cell extract only one end of the polymer is observed elongating. Which of the following could best account for this result? ~ :> tl <t C: 0 'P ~ ·;: (IJ E o > at·o minus 0 end Q. Free Subunit Concentration A) Profilin-like activity was absent inhibiting subunit recycling B) a plus end formin-like complex was functing C) a polymer severing activity was present in the extract D) a plus end capping protein was abundant in the extract E) the TIP in the extract was depleted g3 38) Which of the following is true of the structure of microtubules? A) they are thin flexible filaments B) they have identical critical concentrations at their plus and minus ends C) they are alpha-helical in structure D) they are stiff hollow cylinders - - ---- --E)-they-are-s-ymmetrie--(non-pola-tj i.fl their-organization ~~ 39) Cancer is more prevalent in older organisms because: A) DNA repair enzymes are expressed at low levels in older organisms B) tumor suppressor genes are expressed at low levels in older organisms C) multiple mutations accumulate over time D) metabolism has slowed and cell replication isn't well controlled E) all of the above 1 L 46) COPII subunits are important for: A) pinching vesicles off donor membranes B) initiating the budding of donor membranes C) promoting vesicle fusion with target membranes D) helping vesicles dock at the correct target membrane E) all of the above 97 47) Which of the following is true of both mitochondria and chloroplasts? A) they both perform carbon fixation B) they both consume oxygen and release CO2 C) they both create ATP D) they both contain enzymes for the citric acid cycle E) they both pump protons into the intermembrane space 7 g 48) Where would you be most likely to find a steroid hormone receptor in this diagram A)N B)PM C) Cytosol D)MVB E)TGN 1 / 49) What do detergents do? - - ~)-the-y--degrade-hydrephobi6-f)r0teH1S-by-a-llowrng-specifie-preteol-ysis-oHH1ehar-ged regions:----- B) they increase osmolarity and promote water movement across the plasma membrane C) they act as specific membrane ionophores, allowing protons to pass freely from one side of the membrane to the other D) they solubilize hydrophobic proteins and lipids by including them in micelles E) all of the above

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1 C
2 D
3 B
4 D
5 A
6 A
7 A
8 B
9 E/C
10 C
11 B
12 E
13 A
14 A
15 E...

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