QuestionQuestion

Write a 10 – 15 pages paper (double-spaced) on the topic

The aim of your term paper is to explain the research study or studies:
• what questions were asked
• what was the experimental approach
• what were the results and what do the results mean?
Start with a background to the field: what was previously known, what were the authors of the study trying to find out, and why is this interesting?

Next, go through the main experiments. Rather than including a separate Methods section, with detailed descriptions of the experimental procedures, followed by a Results section, you should describe each experiment separately. For each experiment, as shown by a figure or table in the research paper, answer the following questions:

• What question was the experiment designed to answer?
• What was the experimental procedure? What kind of information (e.g. mRNA levels, enzyme activity, protein binding interactions) does the procedure give?
• What were the results of the experiment?
• What do the results mean?

Outline the experimental design, describing the techniques briefly; don’t include the details of the methods. Show the results, in the form of figures and tables. Finally, discuss what new information was obtained as a result of the experiments; were the original questions answered, and what new questions do the results pose?

Wrap up your term paper by giving an overview of the experiments. What new information did the researchers present? Did they answer their original questions, support their hypothesis? What are their overall conclusions from this study?

You should cite relevant sources in your text and include a list of references at the end of your term paper. See the style guides for references and figures for details on how to include these.

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These solutions may offer step-by-step problem-solving explanations or good writing examples that include modern styles of formatting and construction of bibliographies out of text citations and references. Students may use these solutions for personal skill-building and practice. Unethical use is strictly forbidden.

Introduction:
History of the Lyme disease dates back thousands of years and it was not until the 1980s, that the actual causative agent of the Lyme disease (and the Rocky Mountain Spotted Fever ) came to light under the scientific endurance of Willy Burgdorfer and the causative pathogen came to be known as Borrelia burgdorferi ( 1).The incidence of Lyme disease in the US is increasing and although the causative agent has been discovered in 1980s, there are lacunae in understanding the intricate details of its biochemistry. Additionally, the gene expression as well as reproduction and the biology of Borrelia burgdorferi remain unclear despite several technological advances in the methods of study and the availability of the research tools.
If we consider the spectrum of the molecular tools, then there is sadly a limitation in the use of them for the study of this particular pathogen. It increases more with the focus shifting towards the aspects of study on cellular biology of Borrellia sp and fundamental reason behind the inapplicability of the standard molecular biological tools and also genetic expression studies were not only constrained by the techniques, but also by the unique biology of the pathogen concerned. As far as the genetic analysis is considered, it is more or less cumbersome despite extensive development in the field of study; there is a scarcity of the appropriate molecular markers or reagents suitable for complex cellular organization of Borrelia burgdorferi that is still not completely understood.
For example, it has an unusual morphology and a highly fragmented genome that are thought to be important in the transmission of the bacteria, along with the infectivity and persistence of infection .Borrelia burgdorferi has the most segmented genome of any bacteria and is composed of a linear chromosome of 900kb along with over 20 linear and circular genetic elements or plasmids   of 5kb -60 kb that play critical roles n the life cycle of the organism.(2, 3).The pathogen itself is much different than a normal bacteria and measures...

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