Multiple sequence alignment of the retrieved mutated cancer genes using Clustal W. The gene sequence Dopamine receptor 2 was retrieved from the COSMIC database. These genes were DRD2 (curated gene) DRD2_ENST00000355319 a mutated gene responsible for breasts cancer and Dopamine receptor 3 responsible for breast cancer. The MSA studies revealed the following results:

CLUSTAL 2.1 multiple sequence alignments
DRD2                   ATGGATCCACTGAATCTGTCCTGGTATGATGATGATCTGGAGAGGCAGAACTGGAGCCGG
DRD2_ENST00000355319    ATGGATCCACTGAATCTGTCCTGGTATGATGATGATCTGGAGAGGCAGAACTGGAGCCGG
DRD                   ATGGCATCTCTGAGCCAG--CTGA----GTGGCCACCTGAACTA-CACCTGTGGGGCAGA
                     **** * **** * * ***    ** * *** * ** *** ** *

DRD2                   CCCTTCAACGGGTCAGACGGGAAGGCGGACAGACCCCACTACAACTACTATGCCACACTG
DRD2_ENST00000355319    CCCTTCAACGGGTCAGACGGGAAGGCGGACAGACCCCACTACAACTACTATGCCACACTG
DRD                   GAACTCCACAGGT--GCCAGCCAGGCCCGC-----CCAC-ATGCCTACTATGCCCTCTCC
                           ** ** *** * * * **** * **** * **********
DRD2                   CTCACCCTGCTCATCGCTGTCATCGTCTTCGGCAACGTGCTGGTGTGCATGGCTGTGTCC
DRD2_ENST00000355319    CTCACCCTGCTCATCGCTGTCATCGTCTTCGGCAACGTGCTGGTGTGCATGGCTGTGTCC
DRD                   TACTGCGCGCTCATCCTGGCCATCGTCTTCGGCAATGGCCTGGTGTGCATGGCTGTGCTG
                        * * ******* * *************** * ******************

DRD2                   CGCGAGAAGGCGCTGCAGACCACCACCAACTACCTGATCGTCAGCCTCGCAGTGGCCGAC
DRD2_ENST00000355319    CGCGAGAAGGCGCTGCAGACCACCACCAACTACCTGATCGTCAGCCTCGCAGTGGCCGAC
DRD                   AAGGAGCGGGCCCTGCAGACTACCACCAACTACTTAGTAGTGAGCCTGGCTGTGGCAGAC
                        *** *** ******** ************ * * ** ***** ** ***** ***

DRD2                   CTCCTCGTCGCCACACTGGTCATGCCCTGGGTTGTCTACCTGGAGGTGGTAGGTGAG---
DRD2_ENST00000355319    CTCCTCGTCGCCACACTGGTCATGCCCTGGGTTGTCTACCTGGAGGTGGTAGGTGAG---
DRD                   TTGCTGGTGGCCACCTTGGTGATGCCCTGGGTGGTATACCTGGAGGTGACAGGTGGAGTC
                        * ** ** ***** **** *********** ** ************ *****

DRD2                   TGGAAATTCAGCAGGATTCACTGTGACATCTTCGTCACTCTGGACGTCATGATGTGCACG
DRD2_ENST00000355319    TGGAAATTCAGCAGGATTCACTGTGACATCTTCGTCACTCTGGACGTCATGATGTGCACG
DRD                   TGGAATTTCAGCCGCATTTGCTGTGATGTTTTTGTCACCCTGGATGTCATGATGTGTACA
                     ***** ****** * *** ****** * ** ***** ***** *********** **

DRD2                   GCGAGCATCCTGAACTTGTGTGCCATCAGCATCGACAGGTACACAGCTGTGGCCATGCCC
DRD2_ENST00000355319    GCGAGCATCCTGAACTTGTGTGCCATCAGCATCGACAGGTACACAGCTGTGGCCATGCCC
DRD                   GCCAGCATCCTTAATCTCTGTGCCATCAGCATAGACAGGTACACTGCAGTGGTCATGCCC

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