Long slender bloodstream form T. brucei were incubated on ice with ...

  1. Home
  2. Homework Library
  3. Biology
  4. Biology - Other
  5. Long slender bloodstream form T. brucei were incubated on ice with ...


Long slender bloodstream form T. brucei were incubated on ice with 0.1, 0.5 or 1.0 mg/ml of anti-VSG IgG and then warmed to 37oC for varying time periods (as shown in the table), before the addition of guinea pig serum (GPS) as a source of complement. Trypanosome cell viability was assayed 25 minutes after the addition of the GPS and the percentage of cells lysed by the various treatments determined. Data is shown by graph below (attached).

a) Describe the trends in this data over the time course of the assay and explain the immunological basis for the cell lysis observed.
b) Explain why bloodstream form T. brucei cells pre-incubated at 37oC for 12 minutes prior to addition of GPS are more resistant to lysis than cells pre-incubated for only 1 minute?

    Solution PreviewSolution Preview

    These solutions may offer step-by-step problem-solving explanations or good writing examples that include modern styles of formatting and construction of bibliographies out of text citations and references. Students may use these solutions for personal skill-building and practice. Unethical use is strictly forbidden.

    a) We can see that the concentration of anti-VSG IgG and duration of pre-incubation at 37°C have an impact on the percentage of cell lysed. Separately we can conclude that higher concentration of anti-VSG IgG the more percentage of the cell is lysed. The duration of the pre-incubation period leads to increased viability of cells, or in the other word, the more the pre-incubation period last the less T.brucei cells are going to be killed. When we observe this to parameters together, we see that in low concentration of antibodies for VSG the drop in the percentage of lysed...

    By purchasing this solution you'll be able to access the following files:

    for this solution

    or FREE if you
    register a new account!

    PayPal, G Pay, ApplePay, Amazon Pay, and all major credit cards accepted.

    Find A Tutor

    View available Biology - Other Tutors

    Get College Homework Help.

    Are you sure you don't want to upload any files?

    Fast tutor response requires as much info as possible.

    Upload a file
    Continue without uploading

    We couldn't find that subject.
    Please select the best match from the list below.

    We'll send you an email right away. If it's not in your inbox, check your spam folder.

    • 1
    • 2
    • 3
    Live Chats