Question

1) Aptamers are identified by using in vitro selection processes, such as panning process in phage display technique. Describe synthesis of aptamers.

2) The combination of antibodies together with nanotechnology tools can lead to even higher level of sensitivity. Based on this principle describe mechanism of prostate-specific antigen (PSA) detection using DNA barcode magnetic nanoparticles.

3) Conductive metal wires can be fabricated using Amyloid-forming peptides. Is actin filaments can be used instead?

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1. Aptamers are identified by using in vitro selection processes, such as panning process in phage display technique. Describe synthesis of aptamers.
Aptamers, the small nucleic acid ligands with particular therapeutic functions as well as unambiguous binding affinity to their specific targets, have revolutionized the use of small nucleic acids as therapeutics (Sefah, Shangguan, Xiong, O'Donoghue & Tan, 2010). To achieve these molecules, the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology is utilized (Sun, Zhu, Lu, Rosato, Tan & Zu, 2014). Selex is based on a process involving both repetitive amplification and enrichment. Specifically, the process involves a number of steps. First, a random single stranded DNA (ssDNA) oligonucleotide must be chemically synthesized so that it contains about 1014 to about 1015 random sequences that are also flanked by conserved binding sites for DNA primers (Sun, Zhu, Lu, Rosato, Tan & Zu, 2014)....

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