Subject Biology Biotechnology

Question

A. Use of other computational tool(s) (taught in class or not) that clearly demonstrates improvement of the functional annotation of the PH domain or protein or both - include in both results and discussion along with figure(s)

B. Model and characterize the PH domain from any 2 distant homologs and compare the biophysical properties - include in both results and discussion along with figure(s). Point out unique features of your assigned protein's PH domain compared to the homolog.

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B. Distant homologue used for comparison to human Gab3 PH domain is a homologue from Dictyostelium discoideum, soil-living amoeba belonging to the phylum Amoebozoa. First, the sequence of interest (found previously by using BLAST to search within Dictyostelium discoideum for putative homologues to Q8www8 sequence of Gab3 from human. Serine/threonine kinase with the following sequence was found:
>XP_646888.1 protein serine/threonine kinase [Dictyostelium discoideum AX4]
MSTAPIKHEGFLTKEGGGFKSWKKRWFILKGGDLSYYKTKGELVPLGVIHLNTSGHIKNSDRKKRVNGFEVQTPSRTYFLCSETEEERAKWIEILINERELLLNGGKQPKKSEKVGVADFELLNLVGKGSFGKVIQVRKKDTGEVYAMKVLSKKHIVEHNEVEHTLSERNILQKINHPFLVNLNYSFQTEDKLYFILDYVNGGELFYHLQKDKKFTEDRVRYYGAEIVLALEHLHLSGVIYRDLKPENLLLTNEGHICMTDFGLCKEGLLTPTDKTGTFCGTPEYLAPEVLQGNGYGKQVDWWSFGSLLYEMLTGLPPFYNQDVQEMYRKIMMEKLSFPHFISPDARSLLEQLLERDPEKRLADPNLIKRHPFFRSIDWEQLFQKNIPPPFIPNVKGSADTSQIDPVFTDEAPSLTMAGECALNPQQQKDFEGFTYVAESEHLR
CDD was used to predict...

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