Polyacrylamide Gel Electrophoresis of Gamma Globulin

After performing lab separating gamma globulin on polyacrylamide electrophoresis write a complete lab report including introduction, methods, results, and discussion.

Proteins are composed of amino acids and can be characterized by their shape, size, biological activity, amino acid composition and electrical charge. Different techniques are used to determine these properties of proteins. For example if a protein is an enzyme then we would use an enzyme assay to determine how quickly the protein converts it’s substrate into products1. If we wanted to know the shape of a protein we might use x-ray crystallography. Determining the size or molecular weight of a protein can be done using several techniques, the most common is Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)2. This technique takes advantage of the fact that proteins can be made to be negatively charged and that when a protein is made to be in it’s most negative form a relationship between charge and size can be determined....

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