1. Sheth RU, Yim SS, Wu FL, Wang HH. Multiplex recording of cellula...

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1. Sheth RU, Yim SS, Wu FL, Wang HH. Multiplex recording of cellular events over time on CRISPR biological tape. Science. 2017 Dec 15; 358(6469): 1457-1461.

a) Briefly summarize the scientific discovery or novel findings of the research described in the paper.
Try to limit the use of scientific jargon while still being factually accurate. Make the summary accessible to a wide audience. Limit your response to 150 words.

b) Briefly describe any commercial applications or products that might arise from the discoveries or findings described in the paper.
Try to limit the use of scientific jargon while still being factually accurate. Make the summary accessible to a wide audience. Limit your response to 150 words.

c) Select at least one of the commercial applications or products you described above and estimate the market potential, in quantitative terms, for the selected application(s) or product(s).
If you make assumptions to arrive at your estimate(s), describe these assumptions and provide reference sources. Limit your response to 150 words.

d) Select at least one of the commercial applications or products you described above and describe three (3) competing approaches.
Limit your response to 150 words.

e) List the top three (3) companies that you think would be interested in hearing more about the commercial application or product. Provide a brief rationale for including each target company.
Limit each rationale to 100 words.

f) Select one of three (3) companies you identified above and provide the name, title, company, and email address of a particular person within the company that you think would be interested in hearing about this potential business opportunity. Briefly describe why you think this person is an appropriate business target.


2. Paper:   Dutt A, Joshi C, Ji X, Cardenas J, Okawachi Y, Luke K, Gaeta AL, Lipson M. On-chip dual-comb source for spectroscopy. Science Advances. 2018 Mar 2; 4(3): e1701858.

a) Briefly summarize the scientific discovery or novel findings of the research described in the paper, and include one potential commercial application.
Limit your response to 150 words.

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1. a)
Ans:
This research article by Seth et al describes for the first time the development framework for a “biological tape recorder” at cellular level, that can be described as a microscopic live recorder of signals over time! The authors make use of a CRISPR –Cas9, a natural immune mechanism of common gut microorganism E.coli that also serves as a natural memory of biological events. In what they described a TRACE (Temporal Recording in Arrays by CRISPR expansion) technology, two plasmids are utilized. First a modified plasmid pTrig, which starts replicating to as high as 653 times in just 4hrs after a signal is perceived. Then the second “recording plasmid” pRec that express Cas1 and Cas2 proteins of CRISPER-Cas9 machinery inserts the spacer sequences of pTrig into bacterial genome. Normally, in the absence of any signal it would insert some sequences of pTrig as a “baseline record”, However when there is biological signal, this spacer incorporation is higher and it's unique upon time. Thus, by designing different pTrig spacer sequences for different stimuli (signals) and recording at different time points, we can actually obtain a “signature record” of biological signals perceived by the bacteria at different time points....

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