b) In growing bacteria to use in your cloning project: Streaking bacteria on plates in a 3-way pattern.
c) Cautioning you for gentle inversion (NO vortexing) after adding the P2 buffer in Alkaline lysis method of DNA isolation protocol.
d) Bromophenol blue in the 6x DNA loading dye for running DNA agarose gel electrophoresis
e) Sucrose in the 6x DNA loading dye for running DNA agarose gel electrophoresis
f) Heating the shrimp alkaline phosphatase to 65 °C after treating the linearized plasmid DNA and before mixing the plasmid in a ligation reaction with the gene of interest?
g) Incubating the heat shock-transformed competent bacteria at 37 °C for 30 minutes before plating them onto the selection plates.
h) IPTG in the LB media used in Lac Z alpha complementation selection
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d) Bromophenol is used as a dye in agarose gel electrophoresis. The dye has a negative charge and in agarose gel, it is moving in the same direction and speed as DNA. That helps visualize the movement of the DNA through the gel....