Literature Project Final Draft INSTRUCTIONS Scientific literatur...

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Literature Project Final Draft INSTRUCTIONS

Scientific literature search.
This writing exercise will consist of an outline, final report summarizing a primary scientific paper related to microbial genetics. The paper will be assigned to each student on a date to be announced. It is your responsibility to find the paper via PubMed or library and print it out. You are tasked with reading and studying the paper in detail.

Using the comments and feedback you got from the outline you can begin to write your final report. The final report should be 4 to 5 typed pages in length with double spacing between lines. Font of Times New Roman 12 point size is to be used. Reports can be submitted as either a word document or PDF. Reports handed-in late will result in deduction of final report points (10% deduction per day after due date). Your reports should cover the following four sections with clear subtitles:

Background- Explain why the study was performed. Describe the main point of the article you were assigned, specifically address why the authors perform their study and what prior information motivated the authors to perform their study. You will need to look up and cite two additional references published before and cited by this publication, in order to determine what was known before this study was published and what results encouraged the authors to perform their study.

Methods- Identify the key experiments performed in this study and how they contributed to the main goal of the paper. Do not spend time summarizing growth conditions, strain, and plasmid or primer design. Do not paraphrase the methods section of the paper. Instead you should focus on ONE novel or interesting method. Do not describe the protocol for how to perform the procedure, but rather explain why the procedure is done. Your analysis should cover the overall purpose of the method, why each critical step is performed, what the results look like and how they are interpreted.

Results- Explain the observed results and how they lead the authors to perform further experiments or to make specific conclusions.

Conclusions- Describe the conclusions of the paper. Explain the findings of the paper and how these results contribute to the significance of this work to the field. Describe what YOU think the immediate (short-term) and greater (long-term) impacts of this study and highlight any further research that should be done.

References- You should include a reference section were you reference the main reference (the paper you were assigned), the two references identified in your background search as well as any other references used. References should follow the format below

Author Surname Author Initial. Year Published. Title. Publication Title Volume number:Pages Used.

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Literature Report

Disruption of sitA Compromises Sinorhizobium meliloti for Manganese Uptake Required for Protection against Oxidative Stressâ–¿

Background
Symbiosis of the host plant Medicago sativa with Sinorhizobium meliloti allows the host plant, M .sativa (alfalfa), to use atmospheric nitrogen fixed by the micro symbiont. However, through initial stages of symbiosis with the host plant, the symbiont Sinorhizobium meliloti, must survive an oxidative burst of hydrogen peroxide (H2O2) formed by the host plant for the symbiotic development to continue. S. meliloti encodes a set of enzymes to defend against the host plant ROS response, such as superoxide dismutases, catalases, and alkylhydroperoxidases (Galibert F. et al 2001).

In the experiment the defective mutants involved in S.melitoti symbiosis and oxidative stress protection were screened and identified to determine their roles in symbiosis. In the process a transposon insertion mutation of sitA was isolated and found to encode periplasmic binding of iron/manganese ABC transporter sitABCD. Transposons are DNA elements that can jump from one place in DNA to another; this movement is known as transposition catalyzed by enzymes called transposases (Cohen S. 2019). An interference of transposon sitA causes higher catalase activity and significant decrease in movement and protein level of superoxide dismutase B (sodB). Such decrease is associated with superoxide sensitivity of sitA mutant.

The main goal of the article was to isolate unique genes present in S. meliloti (symbiont) oxidative stress shield and define if these genes play a role in the development of symbiosis. In addition, the goal was to exhibit how all free living phenotypes...

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