QIAamp Extraction
1. How does the DNA adsorb to the column during the purification process?
2. During the QIAamp extraction process, where is the DNA located at step 9? Step 14? Why?
3. Could this procedure be modified for differential extractions (separation of the sperm and non-sperm cells of a mixture)? How?
4. How do you think the protocol would change if the starting material was a bloodstain cloth? A liquid urine sample?

DNA IQ Extraction
5. Can DNA IQ extracts be quantified using gel electrophoresis with Ethidium Bromide staining? Why or why not?
6. What would happen to the DNA if you perform the wash steps in a regular tube rack and not the magnetic stand provided?
7. Compare and contrast QIAamp silica column & DNA IQ DNA extractions.

FTA Extraction
8. During the DNA purification process, how should “contaminated” waste be disposed of? At which step in the purification protocol could you stop treating waste as biohazardous?
9. What is the purpose of the FTA purification reagent?
10. During the FTA extraction process, where is the DNA located at step 2? Step 7? Why?
11. Is DNA purified using the FTA method quantifiable? Why or why not?

QIAcube Robotic Extraction
12. Compare and contrast the QIAamp manual extraction with the QIAcube extraction. How would you expect your results to differ between the two methods?
13. Name and describe two other robotic instruments that will perform DNA extraction. What extraction kits can they be used with and what are some advantages and disadvantages of each? How do they differ from the QIAcube?
14. What are some advantages of automation in Forensic Science?

Additional Conclusions
15. Which extraction method would you use for a touch DNA sample? Explain your reasoning.
16. Which extraction method would you use for a sample containing a mixture of primarily non-human and some human DNA?

QIAamp Extraction 1. How does the DNA adsorb to the column durin...