Question
Capstone Serial Dilution
1. Please write the following conversions in scientific notation. Example 42 μM = 4.2 x 10-⁵ M
13 M = ____________________μM 0.1 μl = ___________________ L
16 mM = ___________________pM 4 ml = ____________________μl

2. Please write the following decimals into scientific notation.
0.001 = 1 x 10 ______
80,000 = 8 x 10 ______

3. Please convert the following numbers into fractions.
24 % = ____________________
32 % = ____________________

4. How many grams of sucrose (molecular weight = 342.3 g/mol) would you dissolve in 750 ml to get a 7% solution?

5. How many grams will you weight to make 400 ml of a 0.6 M solution of a drug that has a molecular weight of 24,000 mg/mmol?

6. How many parts of the original and how parts of diluent are needed for these dilutions?
1: 19 _______ part(s) original + ________ part(s) diluent = ____ total parts
1: 45 ______ part(s) original + ________ part(s) diluent = _____ total parts

7. You have a 15 M stock solution which you would like to dilute 1:5 four times. What is the final concentration of the solution in mM?

8. Solve the following dilutions problems
a. How would you make a 1X solution from a 70X stock? Start with writing the dilution factor, and then list all the parts (see question 6).
b. How would you make a 200 μl of a 5X solution from a 50X stock solution?

9. Assume that you need to dilute a 3 mM solution to a concentration of 1 x 10 –⁶ M. Pipettors that dispense volumes ranging from 10 -100 µl and 100 - 1000 µl volumes and volumetric flasks for measuring 10 ml or 100 ml are available. Only 1.5 ml of diluent is available. You need only 1000 µl of the final solution.
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7. You have a 15 M stock solution which you would like to dilute 1:5 four times. What is the final concentration of the solution in mM?

Ans:
Stock solution concentration is 15 M or 15000 mM
To calculate the final concentration of after series dilutions, multiply the original concentration by the value of each succeeding dilution factor.
= 15000 mM * 1/5 * 1/5 * 1/5 *1/5
= 24 mM
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