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A review of Jinek et al (The Doudna paper) – A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

1) What type of immunity is illustrated by the CRISPR/CAS Systems utilized by bacteria: innate or adaptive? What would be an example of the other type of immunity?
2) How many different types of CRISPR-based systems are found in bacteria and archaea? Upon which type is technology created in this paper based?
3) In Figure 1A, how many non-protein components are used in the assay? Name them.
4) What specific Protospacer Adjacent Motif is found in the “protospacer 2” target DNA?
5) Which strand of DNA is cut by the “HNH” protease domain of Cas9? How is this shown in Figure 2B?
6) Interpret the graph in Figure 3D. First, tell me the overall message of the graph, then explain how that conclusion is reached.
7) What is the key point, in one sentence, of Figure 5?

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1) What type of immunity is illustrated by the CRISPR/CAS Systems utilized by bacteria: innate or adaptive? What would be an example of the other type of immunity?

Adaptive immunity learns to recognize specific features of pathogens while innate immunity (non-specific) recognize generic features of infection. In the proposed research article Bacteria use adaptive immunity against viruses. Example of innate immunity is full virulence of F. novicida in mice as the lipoprotein triggers an innate immune response in the mouse....

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