Transcribed TextTranscribed Text

Question 6. (4 pt) Newly discovered Protease X is found to have protease activity that appears to be identical to that of poliovirus protease 2A. a.) Based on the mechanism of action of poliovirus protease 2A and the data shown in the figure below, what feature is most likely to be present on the mRNAs of genes B and D? b.) Explain the evidence in the data that supports your conclusion that genes B and D most likely have the feature. Control Protease X mRNPs mRNPs 80S 60S 80S 60S MIA Polysomes Polysomes 40S 40S Gene A B C mill Figure legend. In a study of genes A, B, C, and D in a human cervical carcinoma cell D line (HeLa), untreated cells (Control) were Quantitative RT-PCR of fractions compared to cells treated with Protease X. Top panels: Cell lysates from untreated cells (Control) and Protease X-treated cells were loaded on sucrose gradients and the gradients submitted to centrifugation. Fractions were collected and absorbance at 254 nm wavelength light was measured for each fraction. Bottom panels: RNA was then isolated from each gradient fraction and quantitative RT-PCR was performed using primers specific to mRNAs from each of the four genes of interest. The bottom graphs show the relative levels of each mRNA.

Solution PreviewSolution Preview

These solutions may offer step-by-step problem-solving explanations or good writing examples that include modern styles of formatting and construction of bibliographies out of text citations and references. Students may use these solutions for personal skill-building and practice. Unethical use is strictly forbidden.

    By purchasing this solution you'll be able to access the following files:

    for this solution

    PayPal, G Pay, ApplePay, Amazon Pay, and all major credit cards accepted.

    Find A Tutor

    View available Molecular Biology Tutors

    Get College Homework Help.

    Are you sure you don't want to upload any files?

    Fast tutor response requires as much info as possible.

    Upload a file
    Continue without uploading

    We couldn't find that subject.
    Please select the best match from the list below.

    We'll send you an email right away. If it's not in your inbox, check your spam folder.

    • 1
    • 2
    • 3
    Live Chats