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Question 9. (3 pt) Total RNA from a mouse heterozygous for a loss of function mutation in gene X (Mut +/-) was compared to total RNA from a wild type mouse (WT +/+). Results are shown in the figure below. What type of protein is most likely to be encoded by gene X? What specific protein of this type is most likely to be encoded by gene X? Figure legend. 3 Left panels: Northern blot analysis of liver and brain RNAs isolated from a mouse with two normal gene X copies (WT +/+) 30S > and a mouse with one normal copy of gene X and one mutant 2 copy (Mut +/-). The blot was incubated with a probe from the 45S rRNA precursor. 21S 1 18S > Right graph: The relative amount of the 30S and 21S rRNA species was measured by denitometry of the Northern blot and the 30S/21S ratio was calculated. M, Mutant. WT M WT M liver brain liver brain Question 11. (5 pt) Based on the data in the figure below, draw and label diagrams of the structure of the mRNAs including the 5' end, 3' end, relevant regulatory structure and the coding sequence with start codon for the mRNAs that would encode proteins A, C, and D. Control DFO A B C D Western blot of cell lysates from cells cultured in regular growth medium (Control) or in regular growth medium containing desferoxamine (DFO). Gene A mRNA 5' - - 3' Gene C mRNA 5' - - 3' Gene D mRNA. 5' - - 3'

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