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The by reversed phase chromatography is used for the molecules which has some amt of hydrophobic character the process gives excellent recovery and resolution. The actual separation process involves the hydrophobic binding interaction between the solute molecule in the mobile phase and the immobilised hydrophobic ligand, i.e. the stationary phase in simpler words reversible adsorption and desorption of solute molecules with varying degrees of hydrophobicity to a hydrophobic stationary phase....
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