Question

A negatively charged sample would be expected to migrate towards the anode (positively charged electrode). How then is it that species of all charges are detected in capillary electrophoresis at the cathode (negatively charged) end of the system?

In this question you basically should explain the two major forces that are involved in the principles of capillary electrophoresis and explain the principles of the technique in detail. Why are all compounds, no matter if charged or uncharged, moving towards the cathode? There are two principles and please use them.

Solution Preview

This material may consist of step-by-step explanations on how to solve a problem or examples of proper writing, including the use of citations, references, bibliographies, and formatting. This material is made available for the sole purpose of studying and learning - misuse is strictly forbidden.

Electrophoresis involves the movement of electrically charged particles through a buffer system under influence of electric current.

In an electrophoresis system is made by 2 containers of buffer connected with a fine capillary tube. Now under the influence of electrical gradient +vely charged cations moves to –ve electrode (cathode) & -vely charged anions to moves in +ve electrode (anode). The main principle of separation is based on the fact small molecules move faster than larger ones & molecules having higher charges/size ratio travels faster than molecules having move faster than the lower charges/size ration ones . Now the electrophoresis depends on the differential migration of ions under the electrical gradient....

This is only a preview of the solution. Please use the purchase button to see the entire solution

Related Homework Solutions

Analytical Chemistry Problem
Homework Solution
$30.00
Analytical Chemistry
Urine Test
Commercial Test Strip
Cold Symptoms
Antihistamine
Health
Medicine
Drugs
Results
Cocaine
Police
Laboratory
Technology
Analytical Methods
Defense Attorney
Crime
Law
Analytical Chemistry Article Discussion
Homework Solution
$50.00
Analytical Chemistry
Research
Resources
Critical Evaluation
Cannabinoids
Metabolism
Urine
Marijuna Smokers
Concentration Analysis
Samples
Experiments
Matrix Effect
Clinical Study
Biological Fluids
Chromatographic Separation
Analytical Chemistry Samples
Homework Solution
$18.00
Analytical Chemistry
samples
Extraction
Separation
Detection
Blood
Iron Concentration
Aqueous Fluid
PCB Impurities
Methane Concentrations
Age Authenticity
Acidic Environment
Analytical Chemistry: Two Articles Discussion
Homework Solution
$100.00
Analytical Chemistry
Research
Resources
Mass Spectrometer
Precision
Accuracy
LOQ Levels
LC/MS Methods
E2 Derivatization Reagent
SPE
Time Consuming
Periodic Table
Validation
Water Samples
Malted Wax
Low Cost
Devices
Science Classes
Techno
Analytical Chemistry Questions
Homework Solution
$8.00
Analytical Chemistry
Reverse-Phase HPLC
Water
Benzylalcohol
Benzonic Acid
Chromatogram
Retention Time
Compounds
Pertinent Information
Solute Molecule
Get help from a qualified tutor
Live Chats