(i) The title and reference to the work, including a hyperlink where the article can be found
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These solutions may offer step-by-step problem-solving explanations or good writing examples that include modern styles of formatting and construction of bibliographies out of text citations and references. Students may use these solutions for personal skill-building and practice. Unethical use is strictly forbidden.“Measurement of Estradiol in Human Serum by LC-MS/MS Using a Novel EstrogenSpecific Derivatization Reagent” P.
II. Quantitative determination of 17β-estradiol (E2) is important in clinical settings and traditionally it was done by immunoassays. Due to the sensitivity issues and method biasness, researchers moved towards mass spectrometric methods, especially, liquid chromatography tandem mass spectrometry (LC-MS/MS). In this article, an LC/MS/MS method was developed to quantify estradiol and this method uses a novel estrogen specific derivatization reagent, 1,2dimethylimidazole-5-sulfonyl chloride (DMIS). With E2, this method simultaneously quantifies underivatized testosterone (T) and dihydrotestosterone (DHT). Optimum derivatization conditions were investigated and the reaction mixture was analyzed in 9.5 min gradient run. Detection was achieved by a triple quadrupole mass spectrometer with Atmospheric pressure photoionization. Analytical method validation parameters such as linearity, precision, accuracy, extraction recovery, matrix effect, stability, carry-over, selectivity were determined. They also compared the results from this novel derivatization method with the standard LC/MS/MS technique by analyzing 120 samples from a clinical study and certified E2 serum reference materials BCR-576, BCR-577, and BCR-578. The main purpose of this project is to increase the sensitivity of E2 quantification. The limits of quantification (LOQ) were determined to be E2 (0.5pg/mL), T (25pg/mL), and DHT (0.10 ng/mL). Therefore this method was capable of analyzing E2 in samples such as serum from men, children, and postmenopausal women which have E2 concentrations in low pg/mL range....
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