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(15) l. The product ion spectrum shown below was acquired on an ion trap mass spectrometer by collisionally induced dissociation of the doubly charged ion at m/z=389.9. Toe peptide was produced by an in-gel trypic digestion of a Coomassie blue stained protein band in a 2D gel. Ali peaks are normalized to the largest peak in the spectrum. Selective magnification was used over various portions of the spectrum to improve the clarity ofthe spectrum. No additional ions were observed outside the miz range shown. Determine the sequence of the peptide from the information provided. 100 ,- x3 ---.------ xl0-- - -...., .-- --- x3 ---, 518 ,3 261 .2 005..2 233.0 380,9 120 2 500.Z 6.17 2 ~31 2 303 1 147 1 204 1 4760 575 O 458.0 5572 632.3 350 400 miz 450 500 550 600 650 700 (15) 5. Toe product ion spectrum shown below was acquired on an ion trap mass spectrometer by collisionally induced dissociat ion of the doubly charged ion at m/z=78 4.5. The pept ide was produced by an in-gel trypic digestion of a Coomass ie blue stained protein band in a 2D gel. No additional ions were observed outs ide the miz range shown. Determine the sequence of the peptide from the infonnation prov ided. 1 of 4 Spring 20 17 100 1178.5 390.1 - 982.4 e~. .. 11) o e ro 'O § 50 586.1 .o ro 11) ->~ 658.4 276.9 ñi ce 910.3 49 o 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 miz ( 40) 3. Identify the compounds from the four mass spectra given on the following pages . Clearly indicate what mass spectral fe atures are characteristic of the presence ( or absence) of the functional groups identified . Be sure to annotate ali work in order to receive partía! credit. a)MW =l56 100 128 !J.) 156 o ' ,.1 ' 1 " 1 .1 20 3'0 40 !J.) 6'0 70 8'0 so 10' 0 110 130 140 ., 150 160 b)MW=204 100 105 123 77 50 41 51 67 82 o .L¡llhn-,,,11<ll!.,,,,..,,mln,nmflll¡lll,"'"1'=l,,....i~=,,,lln""1"'"""'1"""""'1"""'""="T""""'"1'"='1'"'"'""T"2<"""" 40 50 W 70 00 'iO 100 110 120 130 140 150 1W 170 100 1<;0 200 c) MW= l36 100 50 3? o 10 20 ló ltl /j) 70 80 91 118 ',() 100 110 120 13:l 1 6 d)MW=l 44 10) 29 68 43 2i YJ !jJ 99 41 73 o ~ o 10 20 ~ ,llJ 50 !jJ 70 00 \:O 100 110 120 130 140

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