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1. The pKa values for D (Aspartic Acid) and E (Glutamic Acid) are compared in table 4.1 to estimate the pKa values for the non-standard amino acid shown above. By observing the values for E and D in the table, the side chain for the aspartic acid is one carbon closer (-CH₂) compared to glutamic acid
(-CH₂-CH₂), which is further and the value changes from 3.86 to 4.07. Therefore, if an additional –CH₂ group is added to the structure, the pKa value should change to approximately 4.28....

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