Use the following information for questions 1 3: A B Consider t...

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Use the following information for questions 1 3: A B Consider the reaction above at 25°C where AG°' = +1800 J/mol for the forward reaction. The concentrations at the beginning of the reaction were [A] = 19 mM and [B] = 10 mM. After 1 hour, the concentrations were [A] = 16 mM and [B] = 13 mM. Gas constant = 8.3 J/mol K Question 1 2 pts Calculate the AG of the reaction after 1 hour. Please show your work. (Round final answer to 1 decimal point) HTML Editor B I u A . A Tx X2 +I T Font Sizes Paragraph J Question 2 1 pts Has the reaction reached equilibrium? Explain. HTML Editor B I u A - A Tx T Font Sizes Paragraph p Question 3 2 pts Calculate the equilibrium constant for the above reaction. Please show your work. (Round final answer to 2 decimal points) Don't forget that AG° = -RTInKeq can be rearranged to the following equations: In(Kea)=-AG°/RT = or Keq = e-AG°'/RT HTML Editor B I u A . A Tx +I T Font Sizes . Paragraph Question 4 4 pts Fill in the blanks of the following table. You do not have to show your work. (Round answers 2 decimal points) Acid, Base pH [H*](M) [OH`] (M) or Neutral 4.65 neutral 1.00 x 10 7 1.61 x 10-4 Consider the following peptide for questions 5-9: o o o H H II H II H II - N - N - c - C OH H H H I HCH HCOH HCH HCH o=c HCH HCH HC CH HCH H II HC CH HCH 1 N HH 3 4 Use the pKas from the table of amino acid from Lecture 2 slide 22. Question 5 2 pts What is the primary sequence of the peptide above? Use one letter amino acid code and identify from N-terminus to C-terminus. HTMLEditorE B I u A - A - Ix X x, . ,II Font Sizes Paragraph - vQuestion 6 2 pts Determine the pl of the peptide. Please show your calculations. (Round final answer to 2 decimal point) HTML Editor B I u A . Tx X AT T Font Sizes Paragraph p Question 7 3 pts Would substituting Ala for residue #3 change the pl of the peptide? Explain. HTML Editor B I u A . . Tx X2 AT T Font Sizes Paragraph Question 8 4 pts Which type of noncovalent interaction would the indicated residue of the peptide possibly make at the given pH? Residue 1 @ pH 5.0 [ Select ] Residue 2 @ pH 6.0 [ Select ] Residue 3 @ pH 11.0 [ Select ] Residue 4 @ pH 10.0 [ Select ] Question 9 4 pts Calculate the fraction of C-termini that are protonated at pH 2.5. Please show your work. (Round final answer to 2 decimal points) HTML Editor B I u A . A Tx x² AT T Font Sizes Paragraph Consider the mixture of proteins listed below for questions 10 - 12: Molecular Weight Protein pl (kDa) A 3.1 265 B 6.9 93 C 10.3 96 D 7.1 43 E 8.6 189 Question 10 3 pts You load the protein mix onto a cation exchange column at pH 5. Next, you apply a "washing" step by passing through buffer at pH 5. Finally, for your elution step, you apply a pH gradient starting from pH 2.0 to pH 13.0. (A gradient buffer system allows you to gradually and continuously change the pH of your mobile phase starting from pH 2 up to pH 13). Indicate the order in which proteins will exit the column during the elution step. Please explain your answer. HTML Editor B I u A - A - Tx IF > x² X2 . B C Question 11 2 pts In a separate experiment, you load the original protein mixture onto a size exclusion column. Please indicate the order of elution. HTML Editor B I u A - A Ix EE ,IT T Font Sizes Paragraph p Question 12 3 pts If your protein of interest is protein B, would using cation exchange or size exclusion be completely successful at separating it from the other proteins? Explain both techniques. HTML Editor B I u A . A Tx AT T Font Sizes Paragraph - - Use the following information and chart to answer questions 13 - 16: Hospitals are looking for anyway to cut costs while maintaining quality of care. One large expense is the delivery oxygen gas to patients, which increases tissues oxygenation levels in patients with impaired breathing function. A company is testing an injectable drug that they claim can increase the amount of oxygen to the tissues by binding to hemoglobin. po2 in po2 in tissues lungs 1.0 Hemoglobin (no drug) 0 0.5 4 8 12 16 pO2(kPa) Question 13 1 pts In the chart above, the solid line represents hemoglobin oxygen binding with NO drug treatment. Which curve above represents hemoglobin oxygen binding with drug treatment? Dashed line Dotted Line Question 14 2 pts Explain your choice from question 13. HTML Editor B I u A . A . Ix x² . T Font Sizes Paragraph p Question 15 3 pts How does the drug effect hemoglobin's affinity for O2? Explain. HTML Editor B I u A . A . Ix > . A T Font Sizes Paragraph Question 16 2 pts Which state of hemoglobin would you predict the drug stabilizes, T or R? Explain. HTML EditorE B I u A . A . Ix x² X2 . B IT 114 Font Sizes Paragraph . p

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