QuestionQuestion

Transcribed TextTranscribed Text

2. An enzyme with a Kn of 2.4x10"4 M was assayed at the following substrate con- centrations: (a) 2 x 10-7 M, (b) 6.3 10-3 M, (c) 10-4 M, (d) 10-3 M, and (e) 0.05 M. The velocity observed at 0.05 M was 128 nmoles x liter x min"¹. Calculate the initial velocities at the other substrate concentrations. 3. If the enzyme concentration in practice problem 2 was increased fivefold, what would the initial velocities be at each of the given substrate concentrations? 6. An enzyme with a Kn of 1.2 x 10"4 M was assayed at an initial substrate concentra- tion of 0.02 M. By 30 sec, 2.7 umoles/liter of product had been produced. How much product will be present at (a) 1 min, (b) 95 sec, (c) 3 min, and (d) 5.3 min? (e) What percent of the original substrate will be utilized by the times indicated? 10. The 1/v axis of a reciprocal plot is labeled v"¹: (nmoles x liter 10². The 1/[S] axis is labeled [S]"!: The plot intersects the two axes at "*2" and "-4," respectively. What are Vax and K.? 12. Embryonic liver tissue zyme that catalyzes the reaction S-P. Adul liver also displays S P activity. Some kinex data are shown below. What conclusions you draw concerning the identity of the DE enzymes? (Data for Practice Problem 12) Observed Initial Velocity (umoles Xng [S] Protein x Extract of Adult Extract of Embrysic (M) Liver (E1) Liver (A) 1.67 x 10 1.05 5.00 1.54 6.66 3.33 X 10 1.98 8.00 2.86 10.00 3.78 11.67 5.00 13.33 6.67 15.0 1.67 x 10"4 7.15 15.4 2.0 x 10° - 8.00 16.0 3.0x10" 10.00 17.1

Solution PreviewSolution Preview

These solutions may offer step-by-step problem-solving explanations or good writing examples that include modern styles of formatting and construction of bibliographies out of text citations and references. Students may use these solutions for personal skill-building and practice. Unethical use is strictly forbidden.

    By purchasing this solution you'll be able to access the following files:
    Solution.pdf.

    $50.00
    for this solution

    or FREE if you
    register a new account!

    PayPal, G Pay, ApplePay, Amazon Pay, and all major credit cards accepted.

    Find A Tutor

    View available Biochemistry Tutors

    Get College Homework Help.

    Are you sure you don't want to upload any files?

    Fast tutor response requires as much info as possible.

    Decision:
    Upload a file
    Continue without uploading

    SUBMIT YOUR HOMEWORK
    We couldn't find that subject.
    Please select the best match from the list below.

    We'll send you an email right away. If it's not in your inbox, check your spam folder.

    • 1
    • 2
    • 3
    Live Chats