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2. An enzyme with a Kn of 2.4x10"4 M was assayed at the following substrate con- centrations: (a) 2 x 10-7 M, (b) 6.3 10-3 M, (c) 10-4 M, (d) 10-3 M, and (e) 0.05 M. The velocity observed at 0.05 M was 128 nmoles x liter x min"¹. Calculate the initial velocities at the other substrate concentrations. 3. If the enzyme concentration in practice problem 2 was increased fivefold, what would the initial velocities be at each of the given substrate concentrations? 6. An enzyme with a Kn of 1.2 x 10"4 M was assayed at an initial substrate concentra- tion of 0.02 M. By 30 sec, 2.7 umoles/liter of product had been produced. How much product will be present at (a) 1 min, (b) 95 sec, (c) 3 min, and (d) 5.3 min? (e) What percent of the original substrate will be utilized by the times indicated? 10. The 1/v axis of a reciprocal plot is labeled v"¹: (nmoles x liter 10². The 1/[S] axis is labeled [S]"!: The plot intersects the two axes at "*2" and "-4," respectively. What are Vax and K.? 12. Embryonic liver tissue zyme that catalyzes the reaction S-P. Adul liver also displays S P activity. Some kinex data are shown below. What conclusions you draw concerning the identity of the DE enzymes? (Data for Practice Problem 12) Observed Initial Velocity (umoles Xng [S] Protein x Extract of Adult Extract of Embrysic (M) Liver (E1) Liver (A) 1.67 x 10 1.05 5.00 1.54 6.66 3.33 X 10 1.98 8.00 2.86 10.00 3.78 11.67 5.00 13.33 6.67 15.0 1.67 x 10"4 7.15 15.4 2.0 x 10° - 8.00 16.0 3.0x10" 10.00 17.1

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