QuestionQuestion

The pka of HEPES is 7.55 at 20 degrees celsius and its molecular weight is 238.31 g/mol. Calculate the amounts of HEPES in grams of 1.0 NaOH in mL that would be needed to make 300 mL of 0.2 M HEPES buffer at pH 7.2.

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According to Henderson-Hesselbach equation:
pH = pKa + log [salt]/[base], substituting the values, we get:
7.2 = 7.55 + log (y/x)
Or, log (y/x) = -0.35...
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