QuestionQuestion

You will first prepare a series of protein "standards" for generating a standard curve. You'll
use the standard curve with the spectrophotometer readings for your samples to estimate the levels of protein in your samples.

To make the standards, you' Il be given a vial containing bovine serum albumin (BSA) protein at a concentration of 1 mg/ml. Using this stock solution and ddH2O as a diluent, prepare tubes containing the following concentrations of BSA:

(200 ul total volume each):

Standard 1: 125 ug/ml BSA
Standard 2: 62.5 ug/ml BSA
Standard 3: 31.25 ug/ml BSA
Standard 4: 15.625 ug/ml BSA

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