QuestionQuestion

Transcribed TextTranscribed Text

1. Explain briefly the general principle of sample separation by chromatography. 2. Give the three equations required to calculate R--values, the CV and the ACV. 3. An unknown sample has been applied to 4 different TLC plates. Each TLC plate has been runi in a different mobile phase. The stationary phase of the TLC plates is made of unmodified silica gel. . @ A c D acetone/toluene unknown unknown 1:6 1:3 system system Complete the table using the TLC plates above. A B c D cv cv cv 3 - - ACV 2 ACV3 4. Consider the results obtained in the table under task 3. Summarise your observations and discuss these interms of the quality of the sample separation. 5. Consider the system used in TLC A;Ethanol/CH_Cl, 1:6. a) Which TLC will show a larger change in the R: values, a change to a Ethanol/CH2CI2 1:7 system or Ethanol/CH_CI, 2:6? b) Why? Explain briefly.

Solution PreviewSolution Preview

This material may consist of step-by-step explanations on how to solve a problem or examples of proper writing, including the use of citations, references, bibliographies, and formatting. This material is made available for the sole purpose of studying and learning - misuse is strictly forbidden.

Answer 1: Chromatography is used to separate sample mixture by their continuous distribution between two phases that is stationary and mobile phase.There are four general principle of sample separation by chromatography, these are adsorption (sample is separated between solid stationary and liquid or gaseous mobile phase on the basis of polarity difference of individual component of sample), partition (sample is separated between liquid stationary and liquid or gaseous mobile phase on the basis of difference in partition coefficients of individual components), ion exchange (sample is separated between solid stationary ion exchange...
$38.00 for this solution

PayPal, G Pay, ApplePay, Amazon Pay, and all major credit cards accepted.

Find A Tutor

View available General Chemistry Tutors

Get College Homework Help.

Are you sure you don't want to upload any files?

Fast tutor response requires as much info as possible.

Decision:
Upload a file
Continue without uploading

SUBMIT YOUR HOMEWORK
We couldn't find that subject.
Please select the best match from the list below.

We'll send you an email right away. If it's not in your inbox, check your spam folder.

  • 1
  • 2
  • 3
Live Chats