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Answer 1: Chromatography is used to separate sample mixture by their continuous distribution between two phases that is stationary and mobile phase.There are four general principle of sample separation by chromatography, these are adsorption (sample is separated between solid stationary and liquid or gaseous mobile phase on the basis of polarity difference of individual component of sample), partition (sample is separated between liquid stationary and liquid or gaseous mobile phase on the basis of difference in partition coefficients of individual components), ion exchange (sample is separated between solid stationary ion exchange...

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