QuestionQuestion

Write a lab report about High-Performance Liquid Chromatography (HPLC).
The full lab report for each lab performed must include:
✓ A paragraph introduction to summarize the experiment’s purpose (5 pts)
✓ Brief description of the instrument (main instrument parts and their function)
✓ Procedure – it is given in the lab manual. You should only include changes to the original procedure. Include the original reference. (5 pts)
✓ Results: raw data, calculations or spectroscopic assignments (30 pts)
✓ Conclusion and Error analysis: Restate your results and discuss the possible errors. Suggest how the experiment could be improved (10 pts)

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Introduction
High Performance Liquid Chromatography (HPLC) is often utilized for accurate and precise analysis in industry and research. HPLC has evolved as a powerful method for the separation and purification of chemicals and drugs of diverse nature. The process involves introducing a mixture of compounds in a column and then separating them based on their differential interactions with the stationary phase and the mobile phase. In HPLC, the size of stationary phase is small (less than or equal to 5 microns). Stationary phase of HPLC is packed in a stainless-steel tube and solvent is passed through the system up to several thousand pounds per square inch (psi) pressure. A...
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