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Question 1 Name three ion sources used by the Mass Spectrometry Facility and identify which would be best for a) a polymer, b) a synthetic peptide, and c) a volatile organic compound. Question 2 Name three mass analyzers used in the Mass Spectrometry Facility and describe which are a) high resolution, b) low resolution, or c) can be either. Question 5 Below is a MALDI-TOF spectrum of a polymer. The polymer contains only C and H, and was ionized with Ag+ . Identify the polymer and the end groups. (Use Ag = 107 Da)

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Q1. Three ion sources used by the Mass Spectrometry Facility are electrospray ionization (ESI), Electron Impact (EI) and Matrix-Assisted Laser Desorption Ionization (MALDI). The best ion source for a polymer is MALDI, because a polymer can be a mixture of several compounds, for a synthetic peptide is ESI, because this is relatively pure sample, and for a volatile organic compound is EI...

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