This material may consist of step-by-step explanations on how to solve a problem or examples of proper writing, including the use of citations, references, bibliographies, and formatting. This material is made available for the sole purpose of studying and learning - misuse is strictly forbidden.Each of three questions are carefully analyzed and results are presented below. Short introduction is given about the importance of the selected analyte in infant formula. Then several methods for determination are mentioned and some advantages of the method of choice presented. Then instrumental set-up and chemicals are presented, along with the mobile phase mixture for the chromatography. The detailed procedure for calibration curves is given for taurine and riboflavin because these two chemicals should be quantified. The step-by-step methodology is presented as well as calculation of concentration per gram of infant formula from data obtained from calibration curve.
Question 1 – Quantitatively analyze taurine in the product
Taurine is often considered as amino acid because of its biological significance. It is chemically a sulfur containing amino acid, which makes it very interesting molecule both biologically and analytically
When taurine reacts with bile acids it forms a bile salts that are required for the number of purposes as fat absorption, cell membrane stabilization, neuromodulation, and brain development. Because it is important for the development of neonates and infants, taurine is added as food supplement. The amount of added taurine is therefore controlled. Several methods have been developed in order to determine taurine concentration in food beverages.
The most common methods are based on chromatographic separation. These methods include derivatization. When HPLC with fluorescent detection is used then sample is often derivatized with o-phthaldehyde/mercaptoethanol and the fluorescent molecule is obtained. The other processes include thiamine and fluorescamine. The UV detection at 350 is also possible with derivatization techniques. One of the big disadvantages is very lengthy preparation of sample and complex derivatizations
The proposed analysis extracts taurine from complex sample by simple methods and determines it based on the formation of product that absorbs in UV region.
The instrument used for quantification is HPLC with UV detection. The obtained product absorbs in the UV region. The chemicals needed include taurine, o-phthaldehyde, boric acid, sulfosalicylic acid, sodium hydroxide