Write a lab report on ultra performance liquid chromatography (UPLC)

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1. Title:
Assay of Ibuprofen tablet by UPLC.

2. Introduction:
The experiment aims to analyze a commercially available ibuprofen tablet to ascertain its API content. Ibuprofen is a non-steroidal anti-inflammatory drug (NSAIDs) which is used as a painkiller and also known as an antiplatelet agent. It is a COX inhibitor, and the side effect is also attributed due to non-selectivity between COX-1 and COX-2 inhibition. It is commercially available in the form of tablet alone and with paracetamol combination. It is chemically 2-methyl-4-(2-methylpropyl) benzene acetic acid (Figure 1). 138-140 ºC.

Approval of drug requires the compliances of specifications, and for tablets, it includes the content of active pharmaceutical agent, uniformity, dissolution, disintegration, etc. The drug content in Ibuprofen tablet can be analyzed by UPLC. For this purpose, a series of standard ibuprofen solutions with different concentrations prepared and analyzed by UPLC. A calibration curve was prepared using concentration vs. area under the curve (AUC) which helped in the analysis of Ibuprofen content in the given commercially available tablet.

3. Methodology
The chemicals required and product formed in this experiment is listed in table 1.

Fume hood, conical flask, ice bath, and hot plate.
UPLC, C18 UPLC column, sonicator, 1ml pipette, 5 and 50 mL volumetric flasks, funnel, syringes, syringe filters, HPLC vials.

Preparation of standard solutions
1. Accurately weighed 50 mg of standard ibuprofen transferred to 50 ml volumetric flask using a funnel.
2. A 25 mL of the mixture with the ratio of 60:40 of acetonitrile:0.1% formic acid (in HPLC grade water) was added to the flask up to the mark to get a stock solution of ibuprofen containing 1000 µg/mL.
3. The stock solution was transferred using a 1ml pipette to six different 5 mL volumetric flask with 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5 ml of quantity and labeled as S-1, S-2, S-3, S-4, S-5, and S-6. In each volumetric flak sufficient amount of mixture of 60:40 acetonitrile:0.1% formic acid (in HPLC water) was added to make-up the volume up to the mark. It produced 50, 100, 150, 200, 250 and 300 µg/mL diluted solution of ibuprofen respectively for S1-S6 (Table 2)....

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