QuestionQuestion

Write a lab report on ultra performance liquid chromatography (UPLC)

Solution PreviewSolution Preview

These solutions may offer step-by-step problem-solving explanations or good writing examples that include modern styles of formatting and construction of bibliographies out of text citations and references. Students may use these solutions for personal skill-building and practice. Unethical use is strictly forbidden.

1. Title:
Assay of Ibuprofen tablet by UPLC.

2. Introduction:
The experiment aims to analyze a commercially available ibuprofen tablet to ascertain its API content. Ibuprofen is a non-steroidal anti-inflammatory drug (NSAIDs) which is used as a painkiller and also known as an antiplatelet agent. It is a COX inhibitor, and the side effect is also attributed due to non-selectivity between COX-1 and COX-2 inhibition. It is commercially available in the form of tablet alone and with paracetamol combination. It is chemically 2-methyl-4-(2-methylpropyl) benzene acetic acid (Figure 1). 138-140 ºC.

Approval of drug requires the compliances of specifications, and for tablets, it includes the content of active pharmaceutical agent, uniformity, dissolution, disintegration, etc. The drug content in Ibuprofen tablet can be analyzed by UPLC. For this purpose, a series of standard ibuprofen solutions with different concentrations prepared and analyzed by UPLC. A calibration curve was prepared using concentration vs. area under the curve (AUC) which helped in the analysis of Ibuprofen content in the given commercially available tablet.

3. Methodology
Chemicals
The chemicals required and product formed in this experiment is listed in table 1.

Apparatus
Fume hood, conical flask, ice bath, and hot plate.
UPLC, C18 UPLC column, sonicator, 1ml pipette, 5 and 50 mL volumetric flasks, funnel, syringes, syringe filters, HPLC vials.

Procedure
Preparation of standard solutions
1. Accurately weighed 50 mg of standard ibuprofen transferred to 50 ml volumetric flask using a funnel.
2. A 25 mL of the mixture with the ratio of 60:40 of acetonitrile:0.1% formic acid (in HPLC grade water) was added to the flask up to the mark to get a stock solution of ibuprofen containing 1000 µg/mL.
3. The stock solution was transferred using a 1ml pipette to six different 5 mL volumetric flask with 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5 ml of quantity and labeled as S-1, S-2, S-3, S-4, S-5, and S-6. In each volumetric flak sufficient amount of mixture of 60:40 acetonitrile:0.1% formic acid (in HPLC water) was added to make-up the volume up to the mark. It produced 50, 100, 150, 200, 250 and 300 µg/mL diluted solution of ibuprofen respectively for S1-S6 (Table 2)....

By purchasing this solution you'll be able to access the following files:
Solution.docx and Solution.xlsx.

50% discount

Hours
Minutes
Seconds
$35.00 $17.50
for this solution

or FREE if you
register a new account!

PayPal, G Pay, ApplePay, Amazon Pay, and all major credit cards accepted.

Find A Tutor

View available Pharmaceutical Chemistry Tutors

Get College Homework Help.

Are you sure you don't want to upload any files?

Fast tutor response requires as much info as possible.

Decision:
Upload a file
Continue without uploading

SUBMIT YOUR HOMEWORK
We couldn't find that subject.
Please select the best match from the list below.

We'll send you an email right away. If it's not in your inbox, check your spam folder.

  • 1
  • 2
  • 3
Live Chats