Specific Aim 3: Identify and characterized the molecule, within sku...

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Specific Aim 3: Identify and characterized the molecule, within skullcap, that is responsible for the anti-epileptic effect. It is about identifying a molecule from a plant that can help treat seizures.
Pass the extract of skullcap through LC-MS and administer the collected constituents in isolate to Kindled Sprague Dawley rats to evaluate an anti-seizure effect
Aim 3.1: Isolate Skullcap’s constituents based of their mass/charge ratio and retention times using LC-MS
Aim 3.3: Perform an elemental analysis and obtain a three-dimensional information about the lead molecule using NMR and X-ray crystallography.

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Aim 3.1: Isolate Skullcap’s constituents based of their mass/charge ratio and retention times using LC-MS
Rationale Separation, isolation and identification of novel bioactive molecules, from the complex mixture found in plant material of Skullcap, can be achieved via liquid chromatography coupled with a mass spectrometer.
Experimental design Potential bioactive molecules will first be extracted from the raw plant material of Skullcap...

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