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Post-Lab Questions:

1. What is the purpose of adding the amylase and amyloglucosidase solutions to the popcorn “slurry” prior to fermentation?

2. What process does the popcorn fermentation resemble most: Dry milling or wet milling? Why? What products might we produce from this process?

3. Which feedstock do you predict will yield more ethanol from the fermentation process? Why?

4. List three (3) other raw materials that may be used to produce bioethanol from starch or simple sugars.

5. Why is corn used as the primary feedstock for bioethanol production in the United States?

6. What are challenges associated with making corn ethanol sustainably?

7. What are the advantages of making ethanol from sugarcane?

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What is the purpose of adding the amylase and amyloglucosidase solutions to the popcorn "slurry" before fermentation?

Before the popcorn “slurry” is fermented, amylase and amyloglucosidase are added. Primarily, amylase refers to an enzyme whose role is to catalyze the hydrolysis of starch into sugar. In the production of ethanol, the amylase solution helps to improve the flow ability of the slurry. The solution works by acting on the α (1,4) glycosidic bonds, hence the production of maltose and dextrins. According to Clifford (2019), the amylase solution must be incubated at between 85 and 95 degrees centigrade so it can produce the liquefaction effect. The amyloglucosidase refers to an enzyme that removes units of glucose from liquified starch, albeit systematically, leading to the production of glucose. Usually, the popcorn slurry contains starchy materials. However, Saccharomyces cerevisiae, which is responsible for fermentation, cannot use...

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