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Il Surface plasmon resonance (SPR) is a technique used to monitor molecular interactions in real time. The measurement in arbitrary instrument units is proportional to the concentration of the complex as a function of time, but the conversion between the two is not typically known. In separate runs, the ligand is introduced at time zero at three different free concentrations: 3 nM, 10 nM, and 30 nM. The smoothed data are shown below. Assuming 1:1 binding stoichiometry, estimate the values of Kp, kon, and Koff. 600 500 400 300 3 nM 10 nM 200 30 nM 100 500 1000 1500 2000 Time (seconds)

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