Surface plasmon resonance (SPR) is a technique used to monitor molecular interactions in
real time. The measurement in arbitrary instrument units is proportional to the concentration of
the complex as a function of time, but the conversion between the two is not typically known. In
separate runs, the ligand is introduced at time zero at three different free concentrations: 3 nM,
10 nM, and 30 nM. The smoothed data are shown below. Assuming 1:1 binding stoichiometry,
estimate the values of Kp, kon, and Koff.
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