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In her discussion, Brittany presents samples contamination as the principal challenge while using PCR method to analyze a DNA sample. Before the invention of PCR, scientists used the Restriction Fragment Length Polymorphism (RFLP) for DNA profiling (University of Leicester, 2014). In RFLP, the investigators allowed the sample to form numerous DNA strands on a nitrocellulose and later analyzed using a high-density X-ray film. As such, it was possible to tell a contaminated sample from the different lengths of the DNA fragments....
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