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Conc SK-0V3 BT-20 CRL-2089 SK-BR3 4T1 Free Dox Doxil 100 100 100 100 100 100 100 3 101 105 105 97 107 72 58 10 101 97 102 102 107 58 53 50 110 100 100 104 90 32 52 150 105 106 87 99 71 28 48 200 102 103 40 76 52 30 43 1- Find IC50 VALUES FOR EACH OF THESE CELL LINES 2- Draw a graph on x-axis: conc values in log vesus the y axis of % of control

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Procedure for calculation of IC50:
SK-0V3 Cell viability
The concertation in nM was converted to LogC by : =log10(N1)
The scattered graph is plotted LogC on X axis and Cell viability (% of control) on Y axis
Then click on any data point on the graph, and click on add trend line(after right click)
Check display equation on the graph. Then select regression equation
After this, regression equation with R2 value displayed, which is based on y = mx + C
Here y = % cell viability
x= log of concentration
C = Constant
m= Coefficient

IC50 =...

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