Question

Based on the following instructions, write a pre-lab report, including theses sections: title, purpose/object, materials, and methods.

Köhler Illumination – For Leica DM750P

Steps:
1) Center Lamp Filament:
*Note: Your microscope has a pre-centered lamp. The manufacturer has already centered the lamp for you and you can move on to step 2.
- Insert Bertrand lens and view light filament
- Use the centering screws or rotate lamp in holder to center light
2) Adjust Interpupillary Separation and Focus Specimen:
- Adjust binocular tubes for interpupillary separation
- Place specimen on stage in light path and focus using course and fine adjustment knobs
3) Diopter correction:
- If one eye piece has a reticule, rotate eye lens of that eyepiece until scale is in focus
- While looking ONLY through the eyepiece with reticle, use FINE adjustment to focus on small detail of specimen
- Cover the eyepiece with reticule adjust fine focus of other eyepiece by rotating the eye lens until the specimen details are in fine focus
4) Focus and Center Field Diaphragm:
- Close the field diaphragm
- Focus field diaphragm by adjusting substage condenser up or down with the condenser focus knob.
- Center the condenser to the field diaphragm by turning the centering screws on the condenser.
- Open field diaphragm until it is just outside field of view
5) Adjust Aperture Diaphragm:
- With the Bertrand lens in place, close down aperture diaphragm all the way.
- (For this microscope, the aperture diaphragm should already be in focus. There is no ability to change the focus on the aperture diaphragm.)
- Center the aperture diaphragm by inserting the two centering tools (stored in the back of the microscope) into the slots on either side of the Bertrend lens.
- Manufacturer recommends the aperture diaphragm should be opened so it takes up ~ ¾ of the field of view. [Note: This is simply a good starting adjustment. The aperture diaphragm is constantly adjusted during an examination to achieve either greater resolution or contrast]
- Remove the Bertrand lens
6) Final Check:
- Look through eye pieces of microscope (without Bertrand lens)
- Specimen should be evenly illuminated and in focus

For centering the stage (Not part of Kohler illumination) – Need to center the objective lenses to the rotation of the stage. Insert two centering tools into the two holes in the revolving nosepiece, directly alongside of the objective lens you wish to center. Turn centering tools to cause the center of the stage’s rotation to overlay the center of the reticle’s cross hair.

Köhler Illumination – For Nikon E200

Steps:
1) Center Lamp Filament:
*Note: Your microscope has a pre-centered lamp. The manufacturer has already centered the lamp for you and you can move on to step 2.
- Insert Bertrand lens and view light filament
- Use the centering screws or rotate lamp in holder to center light
2) Adjust Interpupillary Separation and Focus Specimen:
- Adjust binocular tubes for interpupillary separation
- Place specimen on stage in light path and focus using course and fine adjustment knobs
3) Diopter correction:
- If one eye piece has a reticule, rotate eye lens of that eyepiece until scale is in focus
- While looking ONLY through the eyepiece with reticle, use FINE adjustment to focus on small detail of specimen
- Cover the eyepiece with reticule adjust fine focus of other eyepiece by rotating the eye lens until the specimen details are in fine focus
4) Focus and Center Field Diaphragm:
- Close the field diaphragm
- Focus field diaphragm by adjusting substage condenser up or down with the condenser focus knob.
- Use the field diaphragm centering screws to center field diaphragm
- Open field diaphragm until it is just outside field of view
5) Adjust Aperture Diaphragm:
- With the Bertrand lens in place, close down aperture diaphragm until it is in your field of view
- Focus on the aperture diaphragm by using the slider knob directly below the Bertrand lens
- Center the aperture diaphragm using the two chrome knobs behind the Bertrand lens
- Manufacturer recommends the aperture diaphragm should be opened so it takes up ~ ¾ of the field of view. [Note: This is simply a good starting adjustment. The aperture diaphragm is constantly adjusted during an examination to achieve either greater resolution or contrast]
- Remove the Bertrand lens 6) Final Check:
- Look through eye pieces of microscope (without Bertrand lens)
- Specimen should be evenly illuminated and in focus

For centering the stage (Not part of Kohler illumination) – Need to center the rotation of the stage with the reticle’s cross hair. This is accomplished by turning the centering screws behind the stage. Turn the centering screws until the center of the stage’s rotation overlays with the center of the reticle’s cross hair.

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Lab Report
Köhler Illumination

Purpose: the purpose of the lab is to apply Köhler illumination on the example of two compound microscopes: Leica DM750P and Nikon E200. Köhler illumination is designed to create an even illumination of the object and to ensure that the filament of the source of light will not be reflected anywhere in the sight of the microscope.

Materials:
Leica DM750P
Nikon E200
Bertrand lens (optional)
Specimen (will be given)

Method:
First step is to center lamp filament. It is an optional step, since most microscopes have the filament centered by the manufacturer. However, to center it we use the Bertrand lens to view the light filament, and then, using centering screws and rotation of a lamp in the holder we center the light....

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